Tuse Trupti*, Bhise Satish
Department of Pharmacology,
Smt. Kashibai Navale college of Pharmacy, Kondhwa (Bk), Pune, Maharashtra, India
*Address for Corresponding author
Tuse Trupti
Department of Pharmacology,
Smt. Kashibai Navale college of Pharmacy, Kondhwa (Bk), Pune, Maharashtra, India
Abstract
Objective: The zebrafish has been widely used in toxicological studies to evaluate either a single compound or small panels of compounds. This study is aimed at standardizing and validating the zebrafish model with ethanol and DMSO and to have a quick testing method to screen synthetic compounds for primary evaluation of teratogenicity. Material and methods: In this study various concentrations of each solvent to evaluate the teratogenicity in zebrafish were used. The phenotype changes as pericardial edema and yolk sac edema at 96 hpf were observed with ethanol and DMSO. Results and conclusion: Dose dependent phenotype changes as pericardial edema and yolk sac edema were observed with these solvents. Thus, it can be concluded that the zebrafish model could provide a useful tool in the screening of new drugs for treating human diseases. Some solvents act as carrier solvents for water insoluble drugs hence a controlled study of carrier solvents is needed as these solvents alone can have teratogenic potential.
Keywords: Ethanol, DMSO, Zebrafish embryo, solvents, phenotype
