Research Articles

2018  |  Vol: 4(2)  |  Issue: 4(March-April)  |  https://doi.org/10.31024/ajpp.2018.4.2.20

Phytochemically analyzed Chaste tree leaves extracts involving some catheter-associated urinary tract infection causing bacteria

Aashaq Hussain Allaie¹, Ravi Prakash Mishra^1*, Aijaz Ahmad Ganaie¹, Shoket Ali¹, Ranu Singh²

¹Environmental Biotechnology Lab. Department of P.G. Studies & Research in Biological Sciences, R. D. University, Jabalpur, India

²Department of Botany and Microbiology Mata Gujri Mahila Mahavidyalaya (Autonomous), Jabalpur, India

*Corresponding Author

Ravi Prakash Mishra

Environmental Biotechnology Lab. Department of P.G. Studies & Research in Biological Sciences, R. D. University, Jabalpur, (M.P.) India

Abstract

Objective: To make scientific validation of the ethanomedicinal Vitex negundo (Jacq.) in relation to urinary disorders. Methods: Antibacterial study was carried out on clinically isolated Catheter-associated urinary tract infections (CAUTI) infecting bacteria by disc diffusion method. Results: Among the two extracts tested against three different Catheter-associated urinary tract infections (CAUTIs) bacteria, methanolic extract was effective against E. coli with highest inhibition zone (27 mm) followed by P. aeruginosa with inhibition zone (20 mm) and K. pneumoniae with inhibition zone (19 mm), while, hexane extract also shows highest  effective against E. coli with inhibition zone (29 mm) followed by P. aeruginosa with inhibition zone (23 mm) and K. pneumoniae with inhibition zone (16 mm). Preliminary phytochemical screening shows the presence of phytoconstituents viz alkaloids, glycosides, saponins, carbohydrates, proteins, amino acids, flavonoids, steroids, tannins. Conclusion: Based on the present study, along with previous studies, the ethanomedicinal use of Vitex negundo for the treatment of CAUTI has been scientifically validated.

Keywords: Vitex negunda, Phytochemical, Chaste tree, CAUTI