Research Articles

2020  |  Vol: 6(4)  |  Issue: 4 (July- August)  |  https://doi.org/10.31024/ajpp.2020.6.4.2

Bioactive compound isolated from marine Bacillus safensis MB8 and their cytotoxicity potential

Lalitha Perumal^*

Department of Biotechnology, Pondicherry University, Kalapet, Puducherry-605014, India

*Address for Corresponding Author

Dr. Lalitha Perumal

Department of Biotechnology,

Pondicherry University, Kalapet, Pondicherry-605014, India

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Abstract

Background: Marine bacteria represent a potential source for the production of medically useful compounds against cancer, diabetes, inflammation, infection, etc. Objectives: In the present study, isolation, characterization and identification of bioactive compound from marine Bacillus safensis MB8.  Methods: Breast cancer (MCF-7) cell lines to determine the cytotoxicity activity of bioactive compound produced from marine Bacillus safensis MB8. Results: Marine Bacillus safensis MB8 (Genbank accession no. KJ531643) isolated from deep sea sediments of the Bay of Bengal, India, produced a bioactive compound, Bis (2-ethylhexyl) benzene-1,2-dicarboxylate (BEHBD) with a molecular formula of C₂₄H₃₈O₄. The molecular ion peak at m/z 391 (M⁺). The MTT assay based cytotoxicity assessment of BEHBD in MCF-7 cancer cells revealed the IC₅₀ to be 49.8µg/ml.  The acridine-orange and ethidium bromide (AO/EB) staining of the BEHBD treated cancer cells showed typical characteristic of apoptosis such as nuclear condensation, cell shrinkage and formation of apoptotic bodies. Conclusion: Potent cytotoxicity compound of Bis (2-ethylhexyl) benzene-1, 2-dicarboxylate (BEHBD) produced from marine Bacillus safensis MB8. It was undoubtedly confirmed to BEHBD is a proved cytotoxicity compound. This is the first reported to BEHBD produced by marine Bacillus safensis MB8.

Keywords: Marine bacteria, Bacillus safensis, Bis (2-ethylhexyl) benzene-1,2-dicarboxylate, MCF-7, Cytotoxicity activity, AO/EB staining

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Abbreviations

MCF-7                        - Breast cancer

MTT                            - 3-(4,5-Dimethylthiazol-2-Yl)-2,5-Diphenyltetrazolium Bromide

BEHBD                      - Bis (2-ethylhexyl) benzene-1, 2dicarboxylate

AO/EB                         - Acridine-orange/Ethidium bromide

DNA                            - Deoxyribonucleic acid

MALDI-TOF-MS        - Matrix-assisted laser desorption/ionization time of flight mass spectrometry

GTE                             - Glucose Tris EDTA

SDS                             - Sodium dodecyl sulfate

EDTA                          - Ethylene diamine tetraacetic acid

16 rRNA                      - Ribosomal ribonucleic aicd

dNTPs                         - Deoxyribo nucleotide triphosphate

ZMA                           - Zobell marine agar

ZMB                           - Zobell marine broth

TLC                             - Thin layer chromatography

NaCl                            - Sodium chloride

PCR                            - Polymerase chain reaction

MgCl₂                              - Magnesium chloride

NCBI                          - National center for biotechnology information

BLAST                       - Basic local alignment search tool

MEGA                       - Molecular evolutionary genetics analysis

NJ                             - Neighbor-joining

DMSO                        - Dimethyl sulfoxide

HPLC                          - High performance liquid chromatography

FT-IR                          - Fourier transform infrared

NMR                           - Nuclear magnetic resonance

LC-MS                        - Liquid chromatography Mass spectroscopy

GC-MS                       - Gas chromatography -Mass spectrometry

CDCl₃                         - Deuterated chloroform

NCCS                         - National Centre for Cell Science

DMEM                        - Dulbecco’s modified eagle’s medium

FBS                             - Fetal bovine serum

CO₂                             - Carbon dioxide

ELISA                         - Enzyme linked immunosorbent assay

IC₅₀                             - Inhibitory concentration

PBS                             - Phosphate buffered saline

BEPH                          - Bis- (2-ethylhexyl) phthalate

ESI-MS                       - Electrospray ionization - Mass spectrometry

PPDHMP                    - Pyrrole (1, 2, a) pyrazine 1, 4, dione, hexahydro 3-(d-methyl  prophy) 4, 6-Diamidino-2-phenylindole dihydrochloride