Research Articles

2019  |  Vol: 5(1)  |  Issue: 1(January-February)  |  https://doi.org/10.31024/ajpp.2019.5.1.10

In vitro anti-inflammatory and anti-venom activities of aerial parts of Marsilea quadrifolia Linn.

Mohanraj Subramanian^1* Sangameswaran Balakrishnan²

¹The Tamilnadu Dr MGR Medical University, Guindy, Chennai – 600 032, Tamilnadu, India

^1,2SSM College of Pharmacy, Chinniampalayam, Jambai -638 312, Tamilnadu, India

^*Address for Corresponding Author

Mr. S. Mohanraj (M. Pharm.)

Associate  Professor

Department of Pharmacology

SSM College of Pharmacy, Jambai – 638 312, Erode, Tamilnadu, India

Abstract

Objective: To evaluate the in vitro anti-inflammatory and anti-venom effect of ethanolic extract of Marsilea quadrifolia against the Human red blood cell (HRBC) membrane stabilization method, Inhibition of protein denaturation method and Neutralization of anti-coagulation activity. Materials & Methods: The ethanolic extract at different concentrations was incubated with human HRBC suspension and egg albumin in controlled experimental situation and subjected to determination of absorbance and membrane stabilization and viscosity to assess the anti-inflammatory property. Diclofenac sodium was used as the standard drug and with various concentrations was incubated with citrated plasma and determine clotting time to assess anti-venom activity. Results: The results exhibited a concentration reliant inhibition of HRBC membrane stabilization, protein (albumin) denaturation and neutralization of anticoagulant by the Marsilea quadrifolia. The effect of standard drug was found to be little more when compared the test extract. Conclusion: It can be concluded that aerial parts of Marsilea quadrifolia possessed marked in vitro anti-inflammatory and anti-venom effect.

Keywords:  Naja Kaouthia, Diclofenac sodium, Marsilea quadrifolia, anti-inflammatory, anti-venom