Jayalakshmi B.1*, Kruthika L.1, Amruthesh K. N.2
1Post Graduate Department of Botany, Maharani’s Science College for Women, Mysore-570 006, Karnataka, India
2Applied Plant Pathology Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysore-570 006, Karnataka, India
*Address for Corresponding Author
Jayalakshmi B.
Post Graduate Department of Botany, Maharani’s Science College for Women, Mysore-570 006, Karnataka, India
Abstract
Objective: Oxidative stress is a process due to generation of free radicals which trigger cell damage. Antioxidants significantly delay or prevent oxidation of oxidizable substrates. Plants have long been a source of exogenous antioxidants. In this contest the present work to investigate antioxidants properties and phytochemical constituents of Hemidesmus indicus a very important medicinal plant. Material and methods: Different solvent extracts namely petroleum ether, ethyl alcohol and methanol were prepared .These solvent extracts were subjected to Antioxidant assay by DPPH radical scavenging assay, Hydroxyl radical scavenging assay and Nitric oxide scavenging assay. Phytochemical analysis of petroleum ether, methanol, and alcohol extracts was carried out for the detection of active secondary metabolite or different constitutes such as tannins, alkaloids, flavonoids, terpenoids, steroids, carbohydrates, protein and saponins. Results and conclusion: The evaluation of DPPH radical scavenging activity ranged from 25- 34µg/ml. Hydroxyl radical scavenging showed that the methanol and ethanol extract having moderate scavenging activity (32-33 µg/ml) compare to the petroleum ether extract. Nitric oxide radical scavenging activity showed the methanol showed good scavenging activity with IC50 values of 46µg/ml. Hence lower the IC50 value, higher will be the scavenging activity.
Keywords: Antioxidant activity, Hydroxyl radical scavenging, Nitric oxide scavenging, DPPH radical scavenging, Hemidesmus indicus
