Research Articles

2018  |  Vol: 4(3)  |  Issue: 3(May-June)  |  https://doi.org/10.31024/ajpp.2018.4.3.8

Screening, Production and Characterization of extracellular glutaminase free L-Asparaginase producing endo-symbiontic bacteria from the gut of Mugil cephalus (Mullet fish)

Sharmila Rajan^1*, B. Deivasigamani²

¹Ph.D. Research Scholar, Centre for Advanced Studies, Parangipettai, Annamalai University, Parangipettai - 608 502, Tamil Nadu, India.

²Assistant Professor, Faculty of Marine Sciences, CAS in Marine Biology, Annamalai University, Parangipettai - 608 502, Tamil Nadu, India.

*Corresponding Author:

Sharmila Rajan

Centre for Advanced Studies, Parangipettai, Annamalai University, Parangipettai - 608 502, Tamil Nadu, India.

Abstract

Objective: Gut microbes producing L-asparaginase isolated from marine fishes has proven to be one of the best anticancer agent in case of Acute Lymphoblastic Leukemia (ALL). Hence this study focuses on extracting the L-asparaginase producing microbe from the gut of Mugil cephalus. Materials and methods: The microbes were isolated, screened, identified for maximum enzyme production and the medium was subjected to various optimization processes. Results: L-asparaginase activity was detected on the basis of formation of red colour around the colony. The high yielding strain (LAS-8) was grown in Modified Czapek Dox media with different carbon sources and glycerol showed the maximum enzyme production (39.53IU/mg). Optimization with different pH and temperature resulted in maximum production at pH (6.5-7) and temperature 30°C-40°C. The antibiotic sensitivity test was performed in order to identify whether the microbe is susceptible to antibiotics. Conclusion: Findings of this study suggest that the L-asparaginase isolated from the fish gut can be an effective treatment for Acute Lymphoblastic Leukemia (ALL).

Keywords: Enzyme, L-Asparaginase, Mugil cephalus, Acute Lymphoblastic Leukemia (ALL)