Research Articles

2019  |  Vol: 5(4)  |  Issue: 4 (July- August)  |  https://doi.org/10.31024/ajpp.2019.5.4.16

Elephantopus scaber Linn. extract protects against copper ascorbate induced injury to goat heart mitochondria in-vitro: Involvement of antioxidant mechanisms

Sachin M. Hiradeve^1*, Arnab Kumar Ghosh², Sudeshna Paul ², Debasish Bandyopadhyay², Vinod D. Rangari³

¹School of Pharmacy GH Raisoni University, Saikheda Chhindwara M.P., India

²Oxidative Stress and Free Radical Biology Laboratory, Department of Physiology, University of Calcutta University College of Science and Technology 92 APC Road, Kolkata, India-700 009.

³Institute of Pharmaceutical Sciences, Guru Ghasidas Vishwavidyalaya, Koni, Bilaspur, Chhattisgarh, India-495001

*Address for Corresponding Author

Sachin M. Hiradeve

School of Pharmacy GH Raisoni University, Saikheda Chhindwara, M.P., India

Abstract

Objective: Elephantopus scaber Linn., family Asteraceae, is a small herb found in Neotropics, Europe, Asia, Africa and Australia. The plant parts of this herb have been used traditionally for the treatment of a number of diseases in many countries. In the present study, we investigated the antioxidant activities of E. scaber methanolic and aqueous extract of root and aerial part against copper-ascorbate induced toxic injury to mitochondria obtained from goat heart, in vitro. Material and methods: Incubation of isolated cardiac mitochondria with copper-ascorbate resulted in elevated levels of lipid peroxidation and protein carbonylation of the mitochondrial membrane, a reduced level of mitochondrial GSH and altered status of antioxidant enzymes as well as decreased activities of pyruvate dehydrogenase and the Kreb’s cycle enzymes. All of these parameters were protected from being altered when the cardiac mitochondria were co-incubated with E. scaber different extracts, in vitro. Results and conclusion: A significant increase in cardiac mitochondrial LPO level following the incubation of mitochondria with copper ascorbate (63.92%, P < 0.001 vs. control) and protein carbonyl assay (64.78%, P < 0.001 vs. control).  A significant decrease in cardiac mitochondrial reduced GSH content (61.89%, P < 0.001 vs. control), (66.69%, #P < 0.001 vs. control group) in the activity of GPx , (63.91%, #P < 0.001 vs. control group) in the activity of GR  was observed. The result of present study suggested that the E. scaber extract may be used as a potential therapeutic antioxidant agent in future.

Keywords: Elephantopus scaber, copper ascorbate, antioxidant, mitochondria, protein carbonylation