KSSL Ramya1, Tajuddin Shaik2*, B. Ramesh3, Kakaraparthy Ravi Shankar4
1,2Assistant Professor, Malla Reddy College of Pharmacy, Hyderabad, Telangana, India
3Student, Jawaharlal Nehru Technological University, Anantapur, Andhra Pradesh, India
4Principal & Professor, Aditya College of Pharmacy, Andhra Pradesh, India
*Address for Corresponding Author
Tajuddin Shaik
Assistant Professor,
Malla Reddy College of Pharmacy, Hyderabad, Telangana, India
Abstract
Background: Epipremnum aureum is an ayurvedic medicinal plant, leaves and roots of this plants are traditionally used in treat various diseases from ancient times, Herein, we have evaluated its phytochemical composition with antioxidative and anticataract potency of Epipremnum aureum leaves. Objectives: To evaluate effectiveness of Epiprenum aureum ethanolic leaves extract in vitro as an antioxidant and anticataract. To carry out the preliminary screening and free radical scavenging activity of phytochemicals of ethanolic leaves extract of Epipremnum aureum. To evaluate the Invitro and in vivo Anticataract Activity of ethanolic leaves extract of Epiprenum aureum in Glucose induced cataractogenesis using eye lens of Goat and in Galactose induced Cataractogenesis in Rats respectively. Materials and Methods: The antioxidant and anticataract evaluation of the ethanolic extracts of Epipremnum aureum leaves were carried out by using free radical scavenging activity by the Nitric oxide Scavenging Assay, Reducing power method and Phosphomolybdenum antioxidant assay. The invitro anticataract activity was studied on goat eye by comparing there reducing ability of plant extract and standard drug Enalapril in the opacity of cataract lenses. The Galactose model is implemented here to study the in vivo anticataract. By placing the rat on water and Galactose diet for 30 days to induce the cataract and then treated with plant extract. Results: The result of DPPH radical scavenging activity shows a concentration dependent antioxidant activity of Epipremnum aureum which is comparable with a standard ascorbic acid and IC50 was found to be 87.09 µg/ml. The nitric oxide scavenging assay IC50 was found to be 24.5 µg/ml. Phosphomolybdenum anti-oxidant assay at 50µg/ml found to be 0.06±0.23. Aldose reductase AR activity fount to be 97.28 ± 0.0032 in group 1 case. Photographs of Lens in Galactose cataract invivo model clearly indicates the anti-cataract activity.Level of MDA in cataract lenses (group 2) was significantly decreased compared with regular controls (Group 1).The results of invitro and invivo studies indicate that the selected plant extract has a positive effect on the anti-cataract potential, with the opacity of cataract lenses being reduced. Conclusion: Our findings from experiment provide evidence that the crude ethanolic extract of Epipremnum aureum is a potential source of natural antioxidants, and it justify the uses of Epiprenum aureum in folkloric medicines.
Keywords: Epiprenum aureum, phytochemical investigation, antioxidant, anticataract activity
