Jenifer P.1, C. P. Balakrishnan2*, S. Chidambaram Pillai3
1Research Scholar, PG and Research Department of Botany, V.O. Chidambaram College, Tuticorin- 628 008, Affiliation of Manonmaniam Sundaranar University, Abishekapatti, Tirunelveli – 627 012, Tamil Nadu, India
2*Assistant Professor, Department of Botany, Aditanar College of Arts and Science, Virapandianpatnam, Tiruchendur - 628 216, Tamil Nadu, India
3Associate Professor, PG and Research Department of Botany, V.O. Chidambaram College, Tuticorin- 628 008, Tamil Nadu, India
*Address for Corresponding Author
Dr. C. P. Balakrishnan
Assistant Professor
Department of Botany, Aditanar College of Arts and Science,
Virapandianpatnam, Tiruchendur - 628 216, Tamil Nadu, India
Abstract
Objective: The present study was aimed to investigate the quantification of physicochemical and identification of bioactive compound 1, 2 Bezenedicarboxylic acid from methanolic extract of red alga Gracilaria corticata J. Ag. Material and methods: Quantification of physicochemical was carried out according to the standard method. To separate and identify the bioactive constituent by using Column chromatography (CC), GC-MS and NMR were performed. Results: The present study showed that physicochemical parameters such as moisture content, ash value, extractive values and crude fibre were presence at the potential of drug quality. In GC-MS spectrum a major compound revealed that 1, 2-Benzenedicarboxylic acid, mono (2-ethylhexyl) ester (RT 31.54, Peak area 23.15%). The phytochemical compounds are confirmed by NMR (1H and 13C) analysis. Conclusion: It can be concluded that the study was characterized the bioactive constituent present in methanolic extract of this seaweed which will be useful its proper identification.
Keywords: G. corticata, physicochemical, bioactive compound, CC, GC-MS, NMR
