Bhupendra Kumar Dwivedi, Ashish Manigauha*
Technocrats Institute of Technology-Pharmacy, Bhopal, Madhya Pradesh, India
*Address for Corresponding Author:
Prof. Ashish Manigauha
Technocrats Institute of Technology-Pharmacy, Bhopal,
Madhya Pradesh, India
Abstract
Objective: The aim of this works was to estimate the total phenolic and flavonoid content and to evaluate in vitro antioxidant activity and in vivo heptaoprotective effect against Isoniazid induced liver damage in rats, of the flavonoid rich ethanolic fraction of Grewia asiatica Linn. leaves. Material and methods: Extraction of the leaves was done by ethanolic maceration for 48 h and the extract volume was reduced. Total phenolic and flavonoid content was determined in terms of gallic acid and quercetin equivalent respectively. The hepatoprotective effect was evaluated by examining the cross section of the liver and analysis of biochemical parameters involved in liver damage. Results: Total phenolic content in the ethanolic extract of the leaves was found to be 2.15 mg/100 g of the dried extract calculated as gallic acid equivalent (r2=0.998)and the total flavonoids content was found to be 1.65 mg/100 g of the dried extract calculated as quercetin equivalent (r2 = 0.999). The extract was screened by DPPH free radical scavenging activity for its antioxidant potential and was found to be possessing antioxidant potential. The extract was able to significantly reduce the levels of biochemical markers of liver damage viz, ALP, SGOT and SGPT. The histological examination examined clear prevention from injury to the tissue caused by Isoniazid. Conclusion: The antioxidant potential of leaves of the plant may pave way for the formulation of newer plant based antioxidant and anti-ageing neutraceutical product treating many complications of human diseases. The hepatoprotective effect found in the extract may be helpful to prepare newer liver strengthening formulations.
Keywords: Antioxidant, DPPH, free radical, Grewia asiatica, flavonoids, Isoniazid
