Rania Indu1, Anjan Adhikari2*, Piyali Basak3, Tapas Kumar Sur4
1UGC-Senior Research Scholar, Department of Pharmacology, R. G. Kar Medical College, 1, Khudiram Bose Sarani, Kolkata-700004, West Bengal, India
2Associate Professor, Department of Pharmacology, R. G. Kar Medical College, 1, Khudiram Bose Sarani, Kolkata-700004, West Bengal, India
3Director, School of Bioscience and Engineering, Jadavpur University, 188, Raja S.C. Mallick Road, Kolkata-700032, West Bengal, India
4Postdoctoral Fellow, Department of Pharmacology, Institute of Post Graduate Medical Education & Research, 244 A.J.C Bose Road, Kolkata-700020, West Bengal, India
*Address for Corresponding Author
Dr. Anjan Adhikari
Associate Professor, Department of Pharmacology, R. G. Kar Medical College, 1, Kshudiram Bose Sarani, Kolkata-700004, West Bengal, India
Abstract
Objective: Diabetes Mellitus is a metabolic disease affecting multiple organs of the body. Hyperlipidemia is often associated with diabetes. Prescription of hypolipidemics along with anti-diabetics is thus a common practice. These drugs entering the body are metabolized by different organs mainly liver and kidney. Thus it is essential to investigate the effect of concomitant therapy on these organs. Present study tried to assess the effect of concomitant therapy of anti-diabetics and hypolipidemics on different biochemical markers in serum and histological changes in different tissues, in animal models induced with comorbid conditions.
Materials and Methods: Diabetes was induced by Streptozotocin and Nicotinamide and hyperlipidemia was induced by High Fat Diet (HFD) in rats. Comorbid condition of diabetes and hyperlipidemia was mimicked in animal model and were treated with antidiabetic drug Metformin and hypolipidemic drug Atorvastatin for 28 days. Biomarkers in serum and morphological alterations of pancreas, liver and kidney tissues were evaluated. Results: Present study showed increased levels of liver biomarkers in serum of the rats induced with comorbid conditions and treated with concomitant medications. Concomitant medications of Metformin and Atorvastatin in the rats induced with comorbid conditions,also revealed significant increase in creatinine, urea and blood urea nitrogen (BUN) levels to 1.85 ± 0.05mg/dL, 32.4 ± 0.18mg/dL and 15.13 ± 0.08mg/dL, respectively. Pathological changes were also evident in the histological sections of pancreas, liver and kidney tissues in the rats induced with comorbid conditions. Conclusion: Present study thus revealed that concomitant therapy, though essential, is associated with multiple risk.
Keywords: Atorvastatin, diabetes mellitus, histology, hyperlipidemia, in vivo, metformin
