Short Communication

2016  |  Vol: 2(5)  |  Issue: 5 (September-October)

In vitro H+ -K + ATPase inhibitory potential of methanolic extract of Carissa carandas Linn. leaves

Ajay Shukla^1*, Sonia Verma², Ram Bishnoi², C. P. Jain²

¹Department of Pharmacy, Guru Ramdas Khalsa Institute of Science and Technology, Jabalpur, M.P. India

²Department of Pharmaceutical Science, Mohanlal Sukhadia University Udaipur Rajasthan, India

Address for Corresponding Author:

Mr. Ajay Kumar Shukla

Department of Pharmacy,

Guru Ramdas Khalsa Institute of Science and Technology, Jabalpur, M.P. India

Email: ashukla1007@gmail.com

Phone No. +919893735320

 

Abstract

Objective: This study was taken to study in vitro H ⁺ -K ⁺ ATPase inhibitory possible of methanolic acetone extract of Carissa carandas Linn. Leave. Materials and methods: Total phenolic and flavonoid contents from extract were quantified and H ⁺ -K ⁺ ATPase inhibition assay was perform in presence of different concentrations of standard (omeprazole) and methanol extract. Results: Extract showed considerable (P < 0.05) proton pump inhibitory activity in the goat gastric mucosal homogenate which was equivalent to standard. Conclusions: The activities were found to be dose dependent and this study indicates that both methanol-acetone extract of Carissa carandas were found to suppress sheep mucosal H+K+ ATPase activity invitro. So further study is needed to confirm the gastroprotective property of Carissa carandas leave.

Key words: Carisa carandas Linn., H+ K+ ATPase inhibition assay, in vitro