Shiny Ganji1, A. M. Ismail2, Easwari Pasam3*, B. Swathi3
1St Anns College of Pharmaceutical Sciences, Santosh Nagar, Mehdipatnam, Hyderabad, Telangana 500028 India
2Periyar College of Pharmaceutical Sciences, Periyar Centenary Educational Complex, Tiruchirappalli, Tamil Nadu 620021 India
3Priyadarshini College of Pharmaceutical Education and Research, Guntur, Andhra Pradesh, India
*Address for Corresponding Author
Easwari Pasam
Priyadarshini College of Pharmaceutical Education and Research, Guntur, Andhra Pradesh, India
Abstract
Objective: The objective of the study is to evaluate the protective effect of a herbal formulation on the mutagenic effect of Omeprazole in mice. Materials and methods: Four groups of adult Swiss mice (6-8 weeks old) weighing 18-22 gm (n = 6) were used. The normal control group received saline and test group –I animals were treated with Omeprazole (25 mg/mouse, p.o..) to sub group-1 and (50 mg/mouse. p.o.) to sub group-II for five days. To test group - II Omeprazole is administered along vitamin E (50 mg/kg, p.o.) for a period of 35 days. Test group-III has received herbal formulation (1.3 mg/mouse, p.o.) for 35 days. On 35th day the animals were sacrificed by cervical dislocation and sperm head abnormality assay was performed. Results: Omeprazole which was administered in lethal dose has showed a dose dependent mean increase in sperm abnormalities. Omeprazole with vitamin E showed that there is significant decrease in the percentage of abnormal sperm in lower doses of Omeprazole i.e. 25 mg than with 50 mg dose. A similar protection was observed in the group treated with herbal formulation challenged by Omeprazole. Conclusion: Omeprazole is capable of inducing abnormalities in mice in a dose dependent manner perhaps of its unscheduled DNA synthesis. Vitamin E offered protection on the sperm abnormalities of mice due to its anti-oxidant activity. Herbal formulation has also showed a better protection on the mutagenic effect of Omeprazole in mice as compared to that of vitamin E.
Keywords: Mutagenesis, Omeprazole, vitamin E, sperm head abnormality
