V. Madhu Sudarsan, A. Sudheer*, K. Rama Krishna, K. Swathi, P. Sarvasree, M. Ashwini
Department of Pharmacology, Raghavendra Institute of Pharmaceutical Education & Research, Anantapur, A.P.-515721, India
*Address for Correspondence
A. Sudheer
Department of Pharmacology, Raghavendra Institute of Pharmaceutical Education & Research, Anantapur, A.P.-515721, India
Abstra​ct
Objective: The present study was taken up to evaluate the possible protective effect of ethanolic extract of Abelmoschus esculentus. L. against doxorubicin (DOX) induced cardiotoxicity in rats. Material and methods: In this experiment, 30 Albino Wistar rats (250 g) were divided into five groups (n = 6). Control group received distilled water for 10 days. Dox treated group received vehicle for 10 days. The remaining three groups received vitamin C and ethanolic extract of Abelmoschus esculentus (100 and 200 mg/kg, p.o.) for 10 days. Cardiotoxicity was induced by administration of single dose of DOX (10mg/kg i.p.) on 7th day of study. Various biochemical parameters are estimated in serum and heart tissue which includes Creatinine kinase (CK-MB), lactate dehydrogenase (LDH), reduced glutathione (GSH), Super oxide dismutase (SOD) and catalase (CAT) and along with histopathological studies. Results and conclusion: DOX treated rats showed a significant increase in myocardial tissue damage markers such as Creatinine kinase (CK-MB), Lactate dehydrogenase (LDH) and significant declines in the levels of reduced glutathione (GSH), Super oxide dismutase (SOD) and catalase (CAT). All biochemical changes which are brought to normal after oral administration of ethanolic extract of Abelmoschus esculentus at doses 100 and 200mg/kg, p.o for 10 days. Moreover, in this study, we have found that oral administration of Abelmoschus esculentus prevented DOX-induced cardiotoxicity by accelerating heart antioxidant defence mechanisms and membrane stabilizing effect.
Keywords: Abelmoschus esculentus extract, Antioxidants, Cardiotoxicity, Doxorubicin, Histopathology, Myocardial injury markers
