Sateesha S.B.1*, Hirday Narayan Sah1, Rajamma A.J.2
1Department of Pharmaceutics, Acharya & BM Reddy College of Pharmacy, Bengaluru 560107, Karnataka, India
2Department of Pharmacognosy, KLE College of Pharmacy, Bengaluru 560010, Karnataka, India
*Address for Corresponding Author
Dr. Sateesha SB
Department of Pharmaceutics
Acharya & BM Reddy College of Pharmacy, Bengaluru-5600107 Karnataka, India
Abstract
Objective: The objective of the present study was to develop the liposomes of “ferrous ascorbate” as novel formulation of Iron supplement. Material and methods: The Liposomes of ferrous ascorbate was developed by thin film hydration technique. Soya lecithin and cholesterol were used to formulate iron liposome. Iron entrapped liposomes were freeze dried to improve their stability. Compatibility of ferrous ascorbate with lipid materials of the formulation was confirmed by FTIR studies. Formulations were characterized for vesicular size, zeta potential, entrapment efficiency, drug loading efficiency and drug release characteristics. Results: Formulation were found to be monodisperse and highly stable as evidenced by scanning electron microscopy (SEM) and zeta potential measurement. The vesicular size and zeta potential of all the formulations were within the range of 271 to 365nm and -35 to -48 mV respectively. Entrapment efficiency of ferrous ascorbate was found to be 63% w/w to 69% w/w respectively. The total iron content in optimized formulation estimated by atomic absorption spectroscopy was found to be 6.5μg/ml. Drug release pattern was measured using dialysis method and it was found to be steady and sustained for the period of 8h. The formulation was found to be stable during three months under the conditions of 40±2°C/ 75±5% RH. Conclusion: We conclude that the developed iron entrapped liposome can be used as iron supplement to rectify the iron deficiency anemia.
Keywords: Reconstitutable nanoliposome, iron deficiency anemia, liposome of ferrous ascorbate, lyophilized liposome
