Anita S. Wagh*, Santosh R. Butle
School of Pharmacy, S.R.T.M. University, Nanded, Maharashtra, India
*Address for Corresponding Author
Anita S. Wagh
Department of Pharmacognosy,
School of Pharmacy, S. R.T.M University, Nanded, Maharashtra, India.
Abstract
Objective: The aim of the present work was the phytochemical investigation and in vitro evaluation of different extracts of Spathodea campanulata P. Beauv. leaves for anticancer activity. Method: Chloroform, Ethyl acetate, Methanol and Aqueous extracts of Spathodea campanulata P. Beauv. were tested for their anticancer activity on three cancer cell lines i.e. MCF-7 (Breast), HL-60 (Leukemia), and HT-29 (Colon) at various concentrations using Sulphorhodamine B assay. Results: Phytochemical analysis of the plant extracts proves the presence of flavonoid, alkaloids, saponins, tannins and triterpenes. Chloroform, Ethyl acetate and Methanolic extracts showed dose dependent anticancer effect on leukemia (HL-60) cancer cells but these extracts were inactive against breast (MCF-7) and colon (HT-29) cancer cell lines. The activities of these different extracts were compared with the standard drug Adriamycin. Aqueous extract was inactive against all three cell lines. Conclusion: The results of in vitro anticancer activity showed that Spathodea campanulata P. Beauv. plant can be used in the treatment of leukemia.
Keywords: Spathodea campanulata P. Beauv., phytochemicals, anticancer activity, Sulphorhodamine B assay
