Research Articles

2018  |  Vol: 4(3)  |  Issue: 3(May-June)  |  https://doi.org/10.31024/ajpp.2018.4.3.16

In vitro evaluation of methanolic extract of red seaweeds against α-amylase and α-glucosidase enzyme inhibitory activity

Ayyadurai Brabakaran,  Nallamuthu Thangaraju*

Algology Laboratory, CAS in Botany, University of Madras, Guindy Campus, Chennai-600 025, India

*Corresponding author

Dr. N. Thangaraju,

Assistant Professor,

Centre for Advanced studies in Botany, University of Madras, Guindy Campus, Chennai-600025 India

Abstracts

Objective: In the present study, methanolic extract of red seaweeds, Spyridia filamentosa, Grateloupia lithophila and Hypnea musciformis against α- amylase, glucosidase enzyme inhibitory activity were carried out. Materials and methods:  Phytochemical constituents of methanolic extract were qualitatively determined. Antidiabetic activity was evaluated by inhibitory potential of methanolic extract against α-amylase and α- glucosidase by spectrophotometric assays. Results: The crude methanolic extract shows stronger inhibitor effect to α- amylase and α- glucosidase of S. filamentosa (IC₅₀ = 58.02 and 66.06 µg/ml), G. lithophila (IC₅₀ = 53.01 and 58.02 µg/ml) and H. musciformis (IC₅₀ = 48.01 and 51.02 µg/ml), respectively. Phytochemical screening of red seaweed extracts revealed the presence of phenols, protein, lipids and carbohydrates. Conclusion: The result suggest that crude methanolic extract of
S. filamentosa have anti- diabetic potential through inhibition of α- amylase and α- glucosidase.

Keywords: Anti diabetic, Seaweeds, α- amylase, enzymes