Ramchandra Gupta1, Ashish Garg1, Prabhakar Sharma1, Prakash Pandey2
1Department of Pharmacy, Guru Ramdas Khalsa Institute of Science and Technology, Pharmacy, Barela, Jabalpur, M.P. 483001
2Department of Chemistry and Pharmacy, Rani Durgavati Vishwavidyalaya (RDVV), Jabalpur, M.P. 482001.
Corresponding author
Ashish Garg
Department of Pharmacy,
Guru Ramdas Khalsa Institute of Science and Technology, Pharmacy, Barela, Jabalpur, M.P. 483001 India
Abstract
Objective: The present study deals with the phytochemical and biological evaluation of Calliandra haematocephala leaves for its wound healing activity in rats. Materials and methods: Leaves of C. haematocephala were collected and used for size reduction to make powder. Powder leaves of plant (100 gm) were extracted with ethyl acetate by hot continuous extraction process using Soxhlet apparatus. Ethyl acetate extract was screened for chemical constituents and evaluated wound healing effect by incision and excision in vivo wound models. The various antioxidants were also estimated into the healed skin to confirm the antioxidant effect. Results: The ethyl acetate was found most chemical constituent i.e. glycosides, terpenoids, tannins and flavonoids. The 2.5 and 5% ethyl acetate extract ointment of Calliandra haematocephala were subjected for detailed study of wound healing activity using incision wound and excision wound models. Healing effect was confirmed by significant (P< 0.05) increase in tensile strength as well as hydroxproline and protein level of healed skin tissues. The histological studies also support the satisfactory wound healing activity of ethyl alcohol extract of Calliandra haematocephala. Conclusion: Calliandra haematocephala leaves found potent wound healing effect in rats and mechanism of healing can be correlated with its antioxidant property.
Keywords: Wound healing, Calliandra haematocephala, incision, excision, antioxidant
