Mayuri Gurav*, Satish Bhise, Snehal Warghade
Smt. Kashibai Navale College of Pharmacy (Savitribai Phule Pune University), Kondhwa, Pune 411048, M.S., India.
*Corresponding author
Mayuri V. Gurav
Department of Pharmacology, Smt. Kashibai Navale College of Pharmacy, Kondhwa, Pune 411048, M.S., India.
Abstract
Objective: The aim of the present study was to investigate the role of Quercetin in beta cell regeneration in vitro and in vivo. Methods: The research work was initiated with in vitro experiment wherein 3-(4, 5-dimethylthiazol-2-yl) -2, 5-diphenyltetrazolium bromide (MTT) assay was performed using the MIN6 cell line. In vivo study was performed in Streptozocin-induced diabetic Wistar rats and Quercetin (QE) was administered orally in three doses (25, 50, 100 mg/kg). Body weights, serum insulin, blood glucose were measured. At the end of the study animals were sacrificed and histological examination was carried out which included normal histopathology, immunohistochemistry and 5-Bromo-2'-deoxyuridine (BrdU) cell proliferation assay. Results: Streptozocin damaged MIN6 cells showed significantly (P < 0.001) higher viabilities after administration of QE (10μg/ml). QE administration significantly (P < 0.0001) increased body weights as compared to Diabetic Control (DC) group. Administration of QE (50mg/kg) and QE (100mg/kg) significantly (P < 0.0001) decreased fasting blood glucose level and significantly (P < 0.001), (P < 0.0001) increased serum insulin level as compared to DC group. Pancreatic Insulin secretion significantly (P < 0.0001) increased in QE (100mg/kg) group as compared to DC group, also restoration of islets, reduced pancreatic damage with increase in number of β-cells was observed in QE (100mg/kg) group as compared to DC group. The increased number of BrdU positive cells was observed on QE (100mg/kg) administration as compared to DC group. Conclusion: The present study thus confirmed beta cell regeneration using QE.
Keywords: Streptozocin, antibody, diabetes, insulin, MIN6
