Susan Kurian*, Leenamma Joseph, V. S. Josekumar
Department of Zoology, Mar Ivanios College (Autonomous), Thiruvananthapuram-695015, Kerala, India
*Address for Corresponding author
Ms. Susan Kurian
Assistant Professor,
Department of Zoology,
Mar Ivanios College, Thiruvananthapuram, Kerala- 695015. India.
Abstract
Objective: The objective of present study was to evaluate the antimicrobial and phytochemicals successive extracts of the leaves petiole of Artocarpus heterophyllus Lam. Methods: The hexane (AHH), ethyl acetate (AHE) and methanol (AHM) extracts of A. heterophyllus were quantitatively analysed for phytochemicals, viz. total phenolics, flavonoids, alkaloids and carbohydrates following standard method. The antimicrobial activity and minimal inhibitory concentration (MIC) of the plant extracts were examined against five bacterial and two fungal strains using agar well diffusion method and two fold serial dilution methods, respectively. Results: The quantitative phytochemical studies of the crude extracts showed methanol extract with highest phenolic, flavonoids, alkaloids and carbohydrates content which was followed by AHE and AHH extracts. There exists significant mean difference in the microbial activities among various extracts (P> 0.01). The maximum activity was exhibited by methanol extract. The GC-MS analysis of methanol extract identifies different phytocomponents which are biologically active. Conclusion: The present study reveals the presence of components highest in AHM that contribute to the pharmacological activity of the plant.
Keywords: Artocarpus heterophyllus, leaf petiole, phytochemical evaluation, Antimicrobial activity, GC-MS
