Yogesh Halekunche*1, Gopalakrishna Burdipad1, Sujatha Kuppusamy2, Suresh Janadri3
1Department of Pharmacology, R.R.College of Pharmacy, Bengaluru,Karnataka, India.
2Faculty of Pharmacy, Sri RamachandraUniversity, Porur, Chennai, Tamilunadu, India.
3 Department of Pharmacology, Acharya&BM Reddy College of Pharmacy, Bengaluru,
Karnataka, India.
*Address for Corresponding Author:
Mr. Yogesh H S
Assistant Professor
Department of Pharmacology
R. R. College of Pharmacy, Bengaluru,
Karnataka, India - 560 090
Tel: +91-9611133444
Abstract
Objective: To carry out the anti-osteoporotic activity of ethanolic extract of Punica granatum in ovariectomized rat model of osteoporosis at three different dose levels of 100, 300 and 500 mg/kg per day. Materials and methods: Healthy female albino rats were divided into six groups of six animals each. Group Iwas sham operated and served as control. All other groups were ovariectomized. Group II was fed with saline and served as ovariectomized control. Groups III–VI were orally treated with estrogen (2mg/kg) and ethanolic leaves extract of Punica granatum(100,300 and 500 mg/kg) respectively for 90 days. Results: The findings assessed on Punica granatum shows significant (P<0.001) increases in femur length, weight and density, significant (P<0.001) increases in serum calcium, phosphorus and reduction in alkaline phosphatase, tartrate resistant acid phosphatase, osteocalcin whereas urine calcium, creatinine and phosphorous levels were significant (P<0.001) decreasesand histology of femur exhibits restorative progress with increased ossification, mineralization and increased osteoclastic activity. Conclusion: Ethanolic extract of Punica granatum leaves shows anti-osteoporotic effect in OVX rats in dose depended manner.
Keywords: Osteoporosis, Punica granatum, Ovariectomy, Bone mineral density (BMD), Tartrate resistant acid phosphatase (TRAP), Osteocalcin (OC)
