Darshan Angadi1*, Omkarswamy Maradimath2, Ittagi Shanmukha2, Prashanth K. U.2
1Department of Pharmacology, SCS College of Pharmacy, Harapanahalli, Karnataka 583131 India
2Department of Pharamaceutical Chemistry, SCS College of Pharmacy, Harapanahalli, Karnataka 583131 India
*Address for Corresponding Author
Darshan Angadi
Department of Pharmacology
SCS College of Pharmacy, Harapanahalli, Karnataka 583131 India
Abstract
Objectives: The objective of study is to evaluate the Neuroprotective effects of stem bark extract of Calotropis gigantea Linn on experimentally induced neurotoxicity in rats. Material and methods: The various extracts were prepared using pet ether, 70% alcohol and water as solvents. After the completion of extraction, preliminary phytochemical study, quantification of antioxidant phytoconsutients like poly phenols, flavonoids and tannins were determined by spectroscopic method and further confirmed by HPLC method also. The extract rich in flavonoids, tannins and polyphenols (70% ethanolic extract) was selected for antioxidant and pharmacological studies. The 70% alcoholic extract of bark of Calotropis gigantea Linn (70% EEBCG) was subjected to pharmacological studies. The oxidative stress and inflammation has been well known in neurodegenerative disorders. Monosodium glutamate (MSG) and aluminium fluoride (AlF3) produces neuronal degeneration in animals. Oxidative stress mechanisms have been recently proposed in the MSG and AlF3 induced neurotoxicity. The compounds having antioxidant property have been suggested neuroprotection in different experimental models. Ethanolic extract of Calotropis gegantea linn bark (EEBCG) is known for its potent antioxidant activity; hence the present study has been designed to evaluate the neuroprotective effect of EEBCG on MSG and AlF3 models. Results: In the present study, 70% EEBCG showed protection against MSG and AlF3 induced neurotoxicity. The antioxidant property of EEBCG may be responsible for its neuroprotective action.
Keywords: Ficus religiosa Linn; antioxidant: Monosodium glutamate, Aluminium fluoride, excitotoxicity, oxidative stress, neurodegeneration
