Alok Pal Jain1*, Rajesh Singh Pawar2
1Dr. Hari Singh Gour University, Sagar (M.P.) India.
2Pharmacognosy and Phytochemistry Laboratory, VNS Institute of Pharmacy, Neelbud, Bhopal, MP, 462044, India
*Corresponding Author
Dr. Alok Pal Jain
Department of Pharmaceutical Sciences
Dr. Hari Singh Gour University, Sagar (M.P.) India.
Abstract
Objective: Phytochemical screening and HPTLC fingerprinting of different extracts of Alpinia galanga rhizomes. Materials and methods: Powdered dried rhizomes of Alpinia galanga was successively extracted with petroleum ether, chloroform and methanol. All extracts were qualitatively tested for presence of different chemical constituents. All extracts were run on HPTLC plates by using suitable solvent system and reports number of components with Rf values. Results: The tests confirmed the presence of phytosterols, trpenoids and fixed oils in petroleum ether extract. Chloroform extract confirmed the presence of alkaloids, and glycosides while methanol extract give positive test for tannins, flavonoids, amino acids and carbohydrates. Conclusion: The HPTLC method is used for qualitative and quantitative evaluation of herbal drug products. This method will be useful to standardized the product and impurity determination in Alpinia galanga and related products. HPTLC fingerprinting of different extracts were also confirmed the concentration of major compounds present in different extracts. Such finger printing is also applicable in differentiating the species from the adulterant and act as biochemical markers for Alpinia galanga in the pharmaceutical products.
Keywords: HPTLC fingerprinting, Alpinia galangal, phytochemical analysis
