Rajashekar S. Chavan1, Vinuth Chikkamath2, Shanmukha I.2*
1PG Dept of Pharmacology, K L E’s College of Pharmacy, Belgaum, Karnataka, India.
2PG Dept of Pharmacology, S.C.S College of Pharmacy, Harapanahalli, Davanagere 583131
Karnataka, India.
*Corresponding author
Shanmukha I.
PG Dept of Pharmacology, S.C.S College of Pharmacy, Harapanahalli, Davanagere 583131
Karnataka, India.
Abstract
Objective: This study was aimed to determine the quantity of total poly phenol, flavonoids, and tannic acid and, to assess the cerebroprotective potential of Sonchusasper (L) hill against monosodium glutamate (MSG) and aluminium fluoride (AlF3) induced cerebrotoxicity in wistar albino rats. Materials and methods: Quantitative determinations of phytochemicals of 95% ethanolic extract by estimation of total phenolic content and tannins. The albino rats received 95% ethanolic extract at a dose of 250 and 500 mg/kg and ascorbic acid 500 mg/kg (p.o) daily for 7 days. On all the 7 days, MSG (2g/kg, i.p.) was administered one hour before drug treatment and aluminium fluoride (AlF3) at 600 ppm for 7 days in drinking water. During the treatment the rats were observed for neurobehavioral performance for 50minutes daily. Oxidative damage and histopathological analysis of tissue were also assessed. Results: By preliminary phytochemical screening found that 95% alcoholic extract contains glycoside, flavonoid, tannin, alkaloid, saponins etc. It was qualitatively observed the extract contains higher concentration of polyphenol, flavonoid, and tannin components. Extract and ascorbic acid administration significantly improved body weight and attenuated locomotors activity, rota rod and balance beam test performance compared with diseased groups and reversed the enzyme levels to the normal. Conclusion: It was concluded that ethanolic extract of Sonchusasper (L) Hill (EESA) contain significant amount of phenols and tannins and these constituents play an essential role as an antioxidant and cerebroprotective effects in both diseased groups.
Keywords: Sonchusasper (L) Hill, cerebroprotective activity, monosodium glutamate, aluminium fluoride
