Poonam Dogra1, Tanvi1, Nilanjan Ghosh1, Suresh Janadri2, Darshan Raj C.G3, Rajendra S.V*4
1Department of Pharmacology, GRD(PG)IMT,214, Rajpur Road, Dehradun-248009, (Uttarakhand), India
2 Department of Pharmacology, Acharya & BM Reddy College of Pharmacy, Bengaluru-560090 (Karnataka,) India
3Department of Chemistry, PA College of Engineering, Nadupadavu, Near Mangalore University, Mangalore-574153 (Karnataka) India.
4Department of Pharmacology,Krupanidhi College of Pharmacy, Bengaluru-560035, (Karnataka), India.
*Address for Correspondence
Dr. Rajendra S.V. M. Pharm., Ph. D.
Professor & HOD
Department of Pharmacology
Krupanidhi College of Pharmacy
Bengaluru-560035, Karnataka, India
Abstract
Objective: To evaluate the antiproliferative and cell cycle analysis of aqueous extract of Emblica officinalis fruits on human neuroblastoma and colorectal cancer cell lines. Materials and methods: In the first phase of the study, E. officinalis aqueous extract was subjected to phytochemical investigation and following which in vitro antioxidant activities were performed. In the second phase, brine shrimp lethality assay was carried out. In the final phase of the investigation, MTT assay was performed on human neuroblastoma and colorectal cancer cell lines to assess the in vitro cytotoxicity activity of the E. officinalis aqueous extract. Subsequently, cell cycle arrest was assessed by flow cytometry. Results: Phytochemical screening revealed the presence of various vital phytoconstituents. There was significant DPPH free radical scavenging activity. Aqueous extract of the fruits of E. officinalis markedly increases the mortality in brine shrimp lethality assay. Further, the extract exhibited cytotoxicity on human colorectal and neuroblastoma cancer cell lines and cell cycle arrest at G0/G1 phase. Conclusion: It can be concluded that the aqueous extract of fruits of E. officinalis showed antioxidant and anticancer activities and even arrested the cell cycle at G0/G1 phase.
Keywords: Emblica officinalis, brine shrimp lethality assay, MTT assay, cell cycle, flow cytometry
