G. Jyothi Reddy1*, K. Bhaskar Reddy2, G. V. Subba Reddy3
1Research Scholar, JNTUA, Ananthapuramu, Andhra Pradesh, India.
2Sri Venkateswara College of Pharmacy, RVS Nagar, Chittoor, Andhra Pradesh, India
3Department of Chemistry, JNTUA, Pulivendula, Andhra Pradesh, India
*Address for Corresponding Author
G. Jyothi Reddy,
Academic Consultant, Department of Pharmacology,
SVU College of Pharmaceutical Sciences, SV University, Tirupati, 517502, India.
Abstract
Background: Postprandial hyperglycemia (PPHG) has been proposed as an independent risk factor for cardiovascular diseases (CVD). Therefore, control of PPHG is suggested to be important in the management of diabetes mellitus and related secondary complications. Inhibition of α-amylase and α-glucosidase enzymes can be an important antidiabetic approach for reducing PPHG in type 2 diabetes melletus. Acarbose, voglibose and miglitol are the typical examples of inhibitors used in the clinical practice. However, these drugs have major drawbacks. Hence the purpose of the present study is to explore natural and safer α-amylase and α-glucosidase inhibitors. Objectives: To evaluate the in vitro antidiabetic activity of aqueous and methanolic extracts of Feronia elephantum fruit and Paspalum scrobiculatum grains. Materials and methods: aqueous and methanolic extracts of Feronia elephantum fruit and Paspalum scrobiculatum grains were assayed by using α-amylase and α-glucosidase enzyme inhibition assays. Results: All the extracts exhibited a dose-dependent inhibitory activity against and α-glucosidase enzymes. The α-amylase IC50 values of methanolic and aqueous extracts of F.elephantum fruit and P.scrobiculatum grains were 119.698, 167.505, 82.107 and 149.886μg/mL respectively which were comparable to that of standard drug acarbose, 109.722μg/mL. The α-glucosidase IC50 values of methanolic and aqueous extracts of F.elephantum fruit and P.scrobiculatum grains were 66.738, 84.548, 47.297 and 118.351μg/mL respectively, with Acarbose showing an IC50 of 52.645μg/ml. Conclusions: Findings of in vitro antidiabetic assay clearly indicate that F.elephantum fruit and P.scrobiculatum grains possesses considerable inhibitory activity against α-amylases and α-glucosidases, and can be of potential use in diabetic patients.
Keywords: α-amylase, α-glucosidase, Feronia limonia, Paspalum scrobiculatum, invitro antidiabetic
