Mithun Singh Rajput*, Devashish Rathore, Rashmi Dahima
School of Pharmacy, Devi Ahilya Vishwavidyalaya, Takshashila Campus, Khandwa Road, Indore-452001, M.P., India
*Address for Correspondence
Dr. Mithun Singh Rajput,
Postdoctoral Fellow, School of Pharmacy, Devi Ahilya Vishwavidyalaya, Takshashila Campus, Khandwa Road, Indore-452001, M.P., India.
Abstract
Background: Neutrophils are one of the components of innate immune system that defend against infectious microorganisms through various antimicrobial capabilities, including microorganism phagocytosis and by releasing bactericidal agents from intracellular secretory granules into the phagosome or to the cell exterior. Activated neutrophils represent the main source of myeloperoxidase (MPO), superoxide (SO) and subsequently derived oxygen metabolites. Objective: The current study was carried out with the aim to investigate the effect of cardamonin on N-formyl-methionyl-leucyl-phenyl-alanine (fMLP) and opsonized zymosan (OZ) stimulated SO generation in neutrophils and their degranulation measured as MPO release. Materials and Methods: Spectrophotometry was used to evaluate the effect of cardamonin (0.1 to 100.0μmol/l) on OZ (0.5 mg/ml) or fMLP (0.1μmol/l) stimulated SO generation and MPO release in neutrophil cells. Superoxide formation was measured in isolated neutrophils as superoxide dismutase inhibitable reduction of cytochrome c and the activity of MPO was assayed by determining the oxidation of o –dianisidine in the presence of hydrogen peroxide. Results: It is evident from one way ANCOVA followed by post-hoc test that cardamonin dose-dependently (1.0 to 100.0μmol/l) significantly decreased (p < 0.05) SO generation and MPO release after each stimulus (fMLP or OZ). Conclusion: Our results indicate that cardamonin could support resolution of inflammation through decreased activity of neutrophils, i.e. respiratory burst and degranulation by inhibiting MPO release and by decreasing the generation of SO and the subsequently derived reactive oxygen species.
Keywords: Cardamonin, Myeloperoxidase, Neutrophils, PMA, Superoxide generation
